ProSci

FECH Antibody

Product Code:
 
PSI-23-569
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 5
Western blot analysis of extracts of various cell lines, using FECH antibody (23-569) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
2 / 5
3 / 5
Immunohistochemistry of paraffin-embedded rat heart using FECH antibody (23-569) at dilution of 1:100 (40x lens).
4 / 5
Immunohistochemistry of paraffin-embedded mouse lung using FECH antibody (23-569) at dilution of 1:100 (40x lens).
5 / 5
Immunohistochemistry of paraffin-embedded mouse stomach using FECH antibody (23-569) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using FECH antibody (23-569) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat heart using FECH antibody (23-569) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using FECH antibody (23-569) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using FECH antibody (23-569) at dilution of 1:100 (40x lens).

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PSI-23-569-50uL50uL£433.00
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Further Information

Additional Names:
FECH, EPP, FCE
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
The protein encoded by this gene is localized to the mitochondrion, where it catalyzes the insertion of the ferrous form of iron into protoporphyrin IX in the heme synthesis pathway. Mutations in this gene are associated with erythropoietic protoporphyria. Two transcript variants encoding different isoforms have been found for this gene. A pseudogene of this gene is found on chromosome 3.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human FECH (NP_000131.2).
NCBI Gene ID #:
2235
NCBI Official Name:
ferrochelatase
NCBI Official Symbol:
FECH
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 50kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P22830
User NOte:
Optimal dilutions for each application to be determined by the researcher.