ProSci

IL25 Antibody

Product Code:
 
PSI-23-479
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using IL25 antibody (23-479) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.
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Immunohistochemistry of paraffin-embedded human prostate using IL25 antibody (23-479) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse lung using IL25 antibody (23-479) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of human lung cancer cells using IL25 antibody (23-479) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of rat bronchus cells using IL25 antibody (23-479) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using IL25 antibody (23-479) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human prostate using IL25 antibody (23-479) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using IL25 antibody (23-479) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of human lung cancer cells using IL25 antibody (23-479) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat bronchus cells using IL25 antibody (23-479) at dilution of 1:100. Blue: DAPI for nuclear staining.

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PSI-23-479-50uL50uL£433.00
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Further Information

Additional Names:
IL25, interleukin 25, IL17E, interleukin 17E, interleukin-17E, interleukin-25
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 33-177 of human IL25 (NP_073626.1).
NCBI Gene ID #:
64806
NCBI Official Name:
interleukin 25
NCBI Official Symbol:
IL25
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 24kDa
Purification:
Affinity purification
Research Area:
Chemokines & Cytokines, Immunology, Innate Immunity
Swissprot #:
Q9H293
User NOte:
Optimal dilutions for each application to be determined by the researcher.