ProSci

NIN Antibody

Product Code:
 
PSI-23-465
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of Jurkat cells, using NIN antibody (23-465) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
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3 / 5
Immunohistochemistry of paraffin-embedded rat kidney using NIN antibody (23-465) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse lung using NIN antibody (23-465) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using NIN antibody (23-465) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of Jurkat cells, using NIN antibody (23-465) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using NIN antibody (23-465) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using NIN antibody (23-465) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using NIN antibody (23-465) at dilution of 1:100. Blue: DAPI for nuclear staining.

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PSI-23-465-50uL50uL£433.00
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Further Information

Additional Names:
SCKL7, ninein, glycogen synthase kinase 3 beta-interacting protein, hNinein, ninein (GSK3B interacting protein), ninein centrosomal protein
Application Note:
WB: 1:500 - 1:1000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This gene encodes one of the proteins important for centrosomal function. This protein is important for positioning and anchoring the microtubules minus-ends in epithelial cells. Localization of this protein to the centrosome requires three leucine zippers in the central coiled-coil domain. Multiple alternatively spliced transcript variants that encode different isoforms have been reported.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-287 of human NIN (NP_891991.1).
NCBI Gene ID #:
51199
NCBI Official Name:
ninein
NCBI Official Symbol:
NIN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 190kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
Q8N4C6
User NOte:
Optimal dilutions for each application to be determined by the researcher.