ProSci

ABCE1 Antibody

Product Code:
 
PSI-23-432
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Immunofluorescence analysis of U2OS cells using ABCE1 antibody (23-432) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using ABCE1 antibody (23-432) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.

Immunofluorescence analysis of U2OS cells using ABCE1 antibody (23-432) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using ABCE1 antibody (23-432) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.

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PSI-23-432-50uL50uL£433.00
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This product comes from: United States.
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Further Information

Additional Names:
ABCE1, RLI, OABP, ABC38, RNS4I, RNASEL1, RNASELI, ATP-binding cassette, sub-family E (OABP), member 1, RNase L inhibitor, ribonuclease L (2', 5'-oligoisoadenylate synthetase-dependent) inhibitor, HP68, OK/SW-cl.40
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the OABP subfamily. Alternatively referred to as the RNase L inhibitor, this protein functions to block the activity of ribonuclease L. Activation of ribonuclease L leads to inhibition of protein synthesis in the 2-5A/RNase L system, the central pathway for viral interferon action. Two transcript variants encoding the same protein have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 320-599 of human ABCE1 (NP_002931.2).
NCBI Gene ID #:
6059
NCBI Official Name:
ATP-binding cassette, sub-family E (OABP), member 1
NCBI Official Symbol:
ABCE1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 67kDa
Purification:
Affinity purification
Research Area:
Cancer, Immunology, Signal Transduction
Swissprot #:
P61221
User NOte:
Optimal dilutions for each application to be determined by the researcher.