ProSci

TTF2 Antibody

Product Code:
 
PSI-23-156
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot analysis of extracts of various cell lines, using TTF2 antibody (23-156) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
2 / 2
Immunofluorescence analysis of A549 cells using TTF2 antibody (23-156). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using TTF2 antibody (23-156) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
Immunofluorescence analysis of A549 cells using TTF2 antibody (23-156). Blue: DAPI for nuclear staining.

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PSI-23-156-50uL50uL£433.00
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Further Information

Additional Names:
HuF2, ZGRF6, transcription termination factor 2, F2, RNA polymerase II termination factor, human factor 2, lodestar homolog, lodestar protein, transcription release factor 2, transcription termination factor, RNA polymerase II, zinc finger, GRF-type containing 6
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
This gene encodes a member of the SWI2/SNF2 family of proteins, which play a critical role in altering protein-DNA interactions. The encoded protein has been shown to have dsDNA-dependent ATPase activity and RNA polymerase II termination activity. This protein interacts with cell division cycle 5-like, associates with human splicing complexes, and plays a role in pre-mRNA splicing.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human TTF2 (NP_003585.3).
NCBI Gene ID #:
8458
NCBI Official Name:
transcription termination factor 2
NCBI Official Symbol:
TTF2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 130kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q9UNY4
User NOte:
Optimal dilutions for each application to be determined by the researcher.