ProSci

ZFYVE1 Antibody

Product Code:
 
PSI-23-006
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot analysis of extracts of 293T cells, using ZFYVE1 antibody (23-006) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 2
Immunofluorescence analysis of A549 cells using ZFYVE1 antibody (23-006). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of 293T cells, using ZFYVE1 antibody (23-006) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of A549 cells using ZFYVE1 antibody (23-006). Blue: DAPI for nuclear staining.

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PSI-23-006-50uL50uL£433.00
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Further Information

Additional Names:
Zinc finger FYVE domain-containing protein 1, Double FYVE-containing protein 1, SR3, Tandem FYVE fingers-1, ZFYVE1, DFCP1, KIAA1589, TAFF1, ZNFN2A1
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
The FYVE domain mediates the recruitment of proteins involved in membrane trafficking and cell signaling to phosphatidylinositol 3-phosphate-containing membranes. This protein contains two zinc-binding FYVE domains in tandem and is reported to localize to the Golgi apparatus. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ZFYVE1 (NP_067083.1).
NCBI Gene ID #:
53349
NCBI Official Name:
Zinc finger FYVE domain-containing protein 1
NCBI Official Symbol:
ZFYVE1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 87kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q9HBF4
User NOte:
Optimal dilutions for each application to be determined by the researcher.