ProSci

SMAD9 Antibody

Product Code:
 
PSI-23-001
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using SMAD9 antibody (23-001) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded human liver damage using SMAD9 antibody (23-001) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human brain cancer using SMAD9 antibody (23-001) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human kidney using SMAD9 antibody (23-001) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human kidney cancer using SMAD9 antibody (23-001) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using SMAD9 antibody (23-001) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using SMAD9 antibody (23-001) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of A549 cells using SMAD9 antibody (23-001). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using SMAD9 antibody (23-001) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human liver damage using SMAD9 antibody (23-001) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human brain cancer using SMAD9 antibody (23-001) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using SMAD9 antibody (23-001) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using SMAD9 antibody (23-001) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using SMAD9 antibody (23-001) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using SMAD9 antibody (23-001) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using SMAD9 antibody (23-001). Blue: DAPI for nuclear staining.

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PSI-23-001-50uL50uL£433.00
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Further Information

Additional Names:
SMAD9, SMAD, mothers against DPP homolog 9 (Drosophila)], HGNC:6774, MADH6, MADH9, SMAD8A, SMAD8B, MAD (mothers against decapentaplegic, Drosophila) homolog 9, MAD, mothers against decapentaplegic homolog 9, mothers against decapentaplegic homolog 9 (Drosophila), Mothers against decapentaplegic, drosophila, homolog of, 9
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is a member of the SMAD family, which transduces signals from TGF-beta family members. The encoded protein is activated by bone morphogenetic proteins and interacts with SMAD4. Two transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human SMAD9 (NP_005896.1).
NCBI Gene ID #:
4093
NCBI Official Name:
SMAD family member 9
NCBI Official Symbol:
SMAD9
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 45-52kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Signal Transduction, Stem Cell
Swissprot #:
O15198
User NOte:
Optimal dilutions for each application to be determined by the researcher.