ProSci

KDM5A Antibody

Product Code:
 
PSI-22-804
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using KDM5A antibody (22-804) at 1:500 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.
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Western blot analysis of extracts of various cell lines, using KDM5A antibody (22-804) at 1:500 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.

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PSI-22-804-50uL50uL£433.00
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This product comes from: United States.
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Further Information

Additional Names:
RBBP-2, RBBP2, RBP2, lysine-specific demethylase 5A, Jumonji, AT rich interactive domain 1A (RBP2-like), histone demethylase JARID1A, jumonji/ARID domain-containing protein 1A, lysine (K)-specific demethylase 5A, retinoblastoma binding protein 2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a member of the Jumonji, AT-rich interactive domain 1 (JARID1) histone demethylase protein family. The encoded protein plays a role in gene regulation through the histone code by specifically demethylating lysine 4 of histone H3. The encoded protein interacts with many other proteins, including retinoblastoma protein, and is implicated in the transcriptional regulation of Hox genes and cytokines. This gene may play a role in tumor progression.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant protein of human KDM5A
NCBI Gene ID #:
5927
NCBI Official Name:
lysine demethylase 5A
NCBI Official Symbol:
KDM5A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 192kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Transcription
Swissprot #:
P29375
User NOte:
Optimal dilutions for each application to be determined by the researcher.