ProSci

SGCB Antibody

Product Code:
 
PSI-22-602
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 4
Western blot analysis of extracts of various cell lines, using SGCB antibody (22-602) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 4
Immunohistochemistry of paraffin-embedded human prostate using SGCB antibody (22-602) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded human esophagus using SGCB antibody (22-602) at dilution of 1:100 (40x lens).
4 / 4
Immunohistochemistry of paraffin-embedded human stomach using SGCB antibody (22-602) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using SGCB antibody (22-602) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human prostate using SGCB antibody (22-602) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using SGCB antibody (22-602) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using SGCB antibody (22-602) at dilution of 1:100 (40x lens).

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PSI-22-602-50uL50uL£433.00
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Further Information

Additional Names:
SGCB, A3b, LGMD2E, SGC
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100
Background:
This gene encodes a member of the sarcoglycan family. Sarcoglycans are transmembrane components in the dystrophin-glycoprotein complex which help stabilize the muscle fiber membranes and link the muscle cytoskeleton to the extracellular matrix. Mutations in this gene have been associated with limb-girdle muscular dystrophy.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 94-318 of human SGCB (NP_000223.1).
NCBI Gene ID #:
6443
NCBI Official Name:
sarcoglycan, beta (43kDa dystrophin-associated glycoprotein)
NCBI Official Symbol:
SGCB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 45kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
Q16585
User NOte:
Optimal dilutions for each application to be determined by the researcher.