ProSci

NASP Antibody

Product Code:
 
PSI-22-569
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 15
Western blot analysis of extracts of various cell lines, using NASP antibody (22-569) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 15
Immunohistochemistry of paraffin-embedded rat spleen using NASP Antibody (22-569) at dilution of 1:100 (40x lens).
3 / 15
Immunohistochemistry of paraffin-embedded rat stomach using NASP Antibody (22-569) at dilution of 1:100 (40x lens).
4 / 15
Immunohistochemistry of paraffin-embedded human tonsil using NASP Antibody (22-569) at dilution of 1:100 (40x lens).
5 / 15
Immunohistochemistry of paraffin-embedded human appendicitis using NASP Antibody (22-569) at dilution of 1:100 (40x lens).
6 / 15
Immunohistochemistry of paraffin-embedded human brain astrocytoma using NASP Antibody (22-569) at dilution of 1:100 (40x lens).
7 / 15
Immunohistochemistry of paraffin-embedded human breast using NASP Antibody (22-569) at dilution of 1:100 (40x lens).
8 / 15
Immunohistochemistry of paraffin-embedded human breast cancer using NASP Antibody (22-569) at dilution of 1:100 (40x lens).
9 / 15
Immunohistochemistry of paraffin-embedded human stomach using NASP Antibody (22-569) at dilution of 1:100 (40x lens).
10 / 15
Immunofluorescence analysis of HeLa cells using TOM20 antibody (22-476) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
11 / 15
Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using NASP Antibody (22-569) at dilution of 1:100 (40x lens).
12 / 15
Immunohistochemistry of paraffin-embedded human adenomyosis using NASP Antibody (22-569) at dilution of 1:100 (40x lens).
13 / 15
Immunohistochemistry of paraffin-embedded mouse lung using NASP Antibody (22-569) at dilution of 1:100 (40x lens).
14 / 15
Immunohistochemistry of paraffin-embedded mouse liver using NASP Antibody (22-569) at dilution of 1:100 (40x lens).
15 / 15
Immunohistochemistry of paraffin-embedded mouse spleen using NASP Antibody (22-569) at dilution of 1:100 (40x lens).
❮❯

Western blot analysis of extracts of various cell lines, using NASP antibody (22-569) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat spleen using NASP Antibody (22-569) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat stomach using NASP Antibody (22-569) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using NASP Antibody (22-569) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human appendicitis using NASP Antibody (22-569) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human brain astrocytoma using NASP Antibody (22-569) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using NASP Antibody (22-569) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using NASP Antibody (22-569) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using NASP Antibody (22-569) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using TOM20 antibody (22-476) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using NASP Antibody (22-569) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human adenomyosis using NASP Antibody (22-569) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using NASP Antibody (22-569) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using NASP Antibody (22-569) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using NASP Antibody (22-569) at dilution of 1:100 (40x lens).

No additional charges, what you see is what you pay! *

CodeSizePrice
PSI-22-569-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Additional Names:
Nuclear autoantigenic sperm protein, NASP, NASP
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a H1 histone binding protein that is involved in transporting histones into the nucleus of dividing cells. Multiple isoforms are encoded by transcript variants of this gene. The somatic form is expressed in all mitotic cells, is localized to the nucleus, and is coupled to the cell cycle. The testicular form is expressed in embryonic tissues, tumor cells, and the testis. In male germ cells, this protein is localized to the cytoplasm of primary spermatocytes, the nucleus of spermatids, and the periacrosomal region of mature spermatozoa.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 180-449 of human NASP (NP_689511.2).
NCBI Gene ID #:
4678
NCBI Official Name:
Nuclear autoantigenic sperm protein
NCBI Official Symbol:
NASP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 85kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
P49321
User NOte:
Optimal dilutions for each application to be determined by the researcher.