ProSci

YARS Antibody

Product Code:
 
PSI-22-489
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot analysis of extracts of various cell lines, using YARS antibody (22-489) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 2
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug YARS antibody (22-489). Western blot was performed from the immunoprecipitate using YARS antibody (22-489) at a dilition of 1:1000.
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Western blot analysis of extracts of various cell lines, using YARS antibody (22-489) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug YARS antibody (22-489). Western blot was performed from the immunoprecipitate using YARS antibody (22-489) at a dilition of 1:1000.

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PSI-22-489-50uL50uL£433.00
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Further Information

Additional Names:
YARS, YRS, YTS, TYRRS, CMTDIC
Application Note:
WB: 1:500 - 1:2000

IP: 1:50 - 1:200
Background:
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human YARS (NP_003671.1).
NCBI Gene ID #:
8565
NCBI Official Name:
tyrosyl-tRNA synthetase
NCBI Official Symbol:
YARS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 59kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Chemokines & Cytokines, Immunology
Swissprot #:
P54577
User NOte:
Optimal dilutions for each application to be determined by the researcher.