ProSci

XPO5 Antibody

Product Code:
 
PSI-22-488
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 5
Western blot analysis of extracts of various cell lines, using XPO5 antibody (22-488).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 5
Immunohistochemistry of paraffin-embedded human oophoroma using XPO5 antibody (22-488) at dilution of 1:100 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded mouse jejunum using XPO5 antibody (22-488) at dilution of 1:100 (40x lens).
4 / 5
Immunofluorescence analysis of MCF7 cells using XPO5 antibody (22-488).
5 / 5
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug XPO5 antibody (22-488). Western blot was performed from the immunoprecipitate using XPO5 antibody (22-488) at a dilition of 1:1000.
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Western blot analysis of extracts of various cell lines, using XPO5 antibody (22-488).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human oophoroma using XPO5 antibody (22-488) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse jejunum using XPO5 antibody (22-488) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using XPO5 antibody (22-488).
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug XPO5 antibody (22-488). Western blot was performed from the immunoprecipitate using XPO5 antibody (22-488) at a dilition of 1:1000.

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PSI-22-488-50uL50uL£433.00
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Further Information

Additional Names:
Exportin-5, Exp5, Ran-binding protein 21, XPO5, KIAA1291, RANBP21
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100

IP: 1:50 - 1:200
Background:
This gene encodes a member of the karyopherin family that is required for the transport of small RNAs and double-stranded RNA-binding proteins from the nucleus to the cytoplasm. The encoded protein translocates cargo through the nuclear pore complex in a RanGTP-dependent process.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 925-1204 of human XPO5 (NP_065801.1).
NCBI Gene ID #:
57510
NCBI Official Name:
Exportin-5
NCBI Official Symbol:
XPO5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 125kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q9HAV4
User NOte:
Optimal dilutions for each application to be determined by the researcher.