ProSci

SCYL1 Antibody

Product Code:
 
PSI-22-447
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using SCYL1 Antibody (22-447) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
2 / 4
Immunohistochemistry of paraffin-embedded human liver damage using SCYL1 antibody (22-447) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded rat kidney using SCYL1 antibody (22-447) at dilution of 1:100 (40x lens).
4 / 4
Immunofluorescence analysis of MCF-7 cells using SCYL1 antibody (22-447). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using SCYL1 Antibody (22-447) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human liver damage using SCYL1 antibody (22-447) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using SCYL1 antibody (22-447) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using SCYL1 antibody (22-447). Blue: DAPI for nuclear staining.

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PSI-22-447-50uL50uL£433.00
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Further Information

Additional Names:
N-terminal kinase-like protein, Coated vesicle-associated kinase of 90 kDa, SCY1-like protein 1, Telomerase regulation-associated protein, Telomerase transcriptional element-interacting factor, Teratoma-associated tyrosine kinase, SCYL1, CVAK90, GKLP, NTKL, TAPK, TEIF, TRAP
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
This gene encodes a transcriptional regulator belonging to the SCY1-like family of kinase-like proteins. The protein has a divergent N-terminal kinase domain that is thought to be catalytically inactive, and can bind specific DNA sequences through its C-terminal domain. It activates transcription of the telomerase reverse transcriptase and DNA polymerase beta genes. The protein has been localized to the nucleus, and also to the cytoplasm and centrosomes during mitosis. Multiple transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 460-740 of human SCYL1 (NP_065731.3).
NCBI Gene ID #:
57410
NCBI Official Name:
N-terminal kinase-like protein
NCBI Official Symbol:
SCYL1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 110kDa
Purification:
Affinity purification
Research Area:
Neuroscience, Signal Transduction
Swissprot #:
Q96KG9
User NOte:
Optimal dilutions for each application to be determined by the researcher.