ProSci

PPP1R8 Antibody

Product Code:
 
PSI-22-418
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 5
Immunofluorescence analysis of MCF7 cells using PPP1R8 antibody (22-418).
2 / 5
Immunohistochemistry of paraffin-embedded mouse liver using PPP1R8 Antibody (22-418) at dilution of 1:100 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded human breast cancer using PPP1R8 Antibody (22-418) at dilution of 1:100 (40x lens).
4 / 5
Immunohistochemistry of paraffin-embedded rat brain using PPP1R8 Antibody (22-418) at dilution of 1:100 (40x lens).
5 / 5
Western blot analysis of extracts of various cell lines, using PPP1R8 antibody (22-418) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunofluorescence analysis of MCF7 cells using PPP1R8 antibody (22-418).
Immunohistochemistry of paraffin-embedded mouse liver using PPP1R8 Antibody (22-418) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using PPP1R8 Antibody (22-418) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using PPP1R8 Antibody (22-418) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using PPP1R8 antibody (22-418) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.

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PSI-22-418-50uL50uL£433.00
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Further Information

Additional Names:
PPP1R8, ARD-1, ARD1, NIPP-1, NIPP1, PRO2047
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-209 of human PPP1R8 (NP_612568.1).
NCBI Gene ID #:
5511
NCBI Official Name:
protein phosphatase 1, regulatory subunit 8
NCBI Official Symbol:
PPP1R8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 40kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
Q12972
User NOte:
Optimal dilutions for each application to be determined by the researcher.