ProSci

P2RX4 Antibody

Product Code:
 
PSI-22-402
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot analysis of extracts of various cell lines, using P2RX4 antibody (22-402) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 2
Immunofluorescence analysis of MCF7 cells using P2RX4 antibody (22-402). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using P2RX4 antibody (22-402) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunofluorescence analysis of MCF7 cells using P2RX4 antibody (22-402). Blue: DAPI for nuclear staining.

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PSI-22-402-50uL50uL£433.00
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Further Information

Additional Names:
purinoceptor P2X4, purinergic receptor P2X4, purinergic receptor P2X ligand-gated ion channel 4, P2X receptor, subunit 4, P2X purinoceptor 4, ATP-gated cation channel protein, ATP receptor, purinergic receptor P2X, ligand-gated ion channel, 4, P2RX4, P2X4R, P2X4
Application Note:
WB: 1:500 - 1:2000

IF: 1:10 - 1:100
Background:
The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel with high calcium permeability. The main pharmacological distinction between the members of the purinoceptor family is the relative sensitivity to the antagonists suramin and PPADS. The product of this gene has the lowest sensitivity for these antagonists. Multiple alternatively spliced transcript variants, some protein-coding and some not protein-coding, have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 55-338 of human P2RX4 (NP_002551.2).
NCBI Gene ID #:
5025
NCBI Official Name:
purinergic receptor P2X, ligand-gated ion channel, 4
NCBI Official Symbol:
P2RX4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 62kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Neuroscience
Swissprot #:
Q99571
User NOte:
Optimal dilutions for each application to be determined by the researcher.