ProSci

HSPH1 Antibody

Product Code:
 
PSI-22-359
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using HSPH1 antibody (22-359) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
2 / 7
Immunohistochemistry of paraffin-embedded rat spleen using HSPH1 antibody (22-359) at dilution of 1:100 (40x lens).
3 / 7
Immunohistochemistry of paraffin-embedded rat fallopian tube using HSPH1 antibody (22-359) at dilution of 1:100 (40x lens).
4 / 7
Immunohistochemistry of paraffin-embedded human tonsil using HSPH1 antibody (22-359) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human vermiform appendix using HSPH1 antibody (22-359) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse spinal cord using HSPH1 antibody (22-359) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using HSPH1 antibody (22-359) at dilution of 1:100 (40x lens).
❮❯

Western blot analysis of extracts of various cell lines, using HSPH1 antibody (22-359) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat spleen using HSPH1 antibody (22-359) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat fallopian tube using HSPH1 antibody (22-359) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using HSPH1 antibody (22-359) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human vermiform appendix using HSPH1 antibody (22-359) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using HSPH1 antibody (22-359) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using HSPH1 antibody (22-359) at dilution of 1:100 (40x lens).

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PSI-22-359-50uL50uL£433.00
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Further Information

Additional Names:
Heat shock protein 105 kDa, Antigen NY-CO-25, Heat shock 110 kDa protein, HSPH1, HSP105, HSP110, KIAA0201
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a member of the heat shock protein 70 family of proteins. The encoded protein functions as a nucleotide exchange factor for the molecular chaperone heat shock cognate 71 kDa protein (Hsc70). In addition, this protein plays a distinct but related role as a holdase that inhibits the aggregation of misfolded proteins, including the cystic fibrosis transmembrane conductance regulator (CFTR) protein. Elevated expression of this protein has been observed in numerous human cancers.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 659-858 of human HSPH1 (NP_006635.2).
NCBI Gene ID #:
10808
NCBI Official Name:
Heat shock protein 105 kDa
NCBI Official Symbol:
HSPH1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 120kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q92598
User NOte:
Optimal dilutions for each application to be determined by the researcher.