ProSci

GPAM Antibody

Product Code:
 
PSI-22-348
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot analysis of extracts of various cell lines, using GPAM antibody (22-348) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 2
Immunohistochemistry of paraffin-embedded mouse heart using GPAM antibody (22-348) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using GPAM antibody (22-348) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded mouse heart using GPAM antibody (22-348) at dilution of 1:100 (40x lens).

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PSI-22-348-50uL50uL£433.00
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Further Information

Additional Names:
GPAT1 Antibody: GPAT, GPAT1, KIAA1560, Glycerol-3-phosphate acyltransferase 1, mitochondrial, GPAT-1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IP: 1:50 - 1:200
Background:
This gene encodes a mitochondrial enzyme which prefers saturated fatty acids as its substrate for the synthesis of glycerolipids. This metabolic pathway's first step is catalyzed by the encoded enzyme. Two forms for this enzyme exist, one in the mitochondria and one in the endoplasmic reticulum. Two alternatively spliced transcript variants have been described for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 594-828 of human GPAM (NP_001231878.1).
NCBI Gene ID #:
57678
NCBI Official Name:
glycerol-3-phosphate acyltransferase, mitochondrial
NCBI Official Symbol:
GPAM
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 94kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
Q9HCL2
User NOte:
Optimal dilutions for each application to be determined by the researcher.