ProSci

ASF1A Antibody

Product Code:
 
PSI-22-288
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot analysis of extracts of mouse pancreas, using ASF1A Antibody (22-288) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 20s.
2 / 2
Immunofluorescence analysis of HeLa cells using ASF1A antibody (22-288). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of mouse pancreas, using ASF1A Antibody (22-288) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 20s.
Immunofluorescence analysis of HeLa cells using ASF1A antibody (22-288). Blue: DAPI for nuclear staining.

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PSI-22-288-50uL50uL£433.00
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Further Information

Additional Names:
ASF1A, ASF1 anti-silencing function 1 homolog A (S. cerevisiae), RP3-329L24.1, CGI-98, CIA, DKFZP547E2110, HSPC146, ASF1 anti-silencing function 1 homolog A, CCG1-interacting factor A, anti-silencing function 1A
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:100
Background:
This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-204 of human ASF1A (NP_054753.1).
NCBI Gene ID #:
25842
NCBI Official Name:
anti-silencing function 1A histone chaperone
NCBI Official Symbol:
ASF1A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 23kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q9Y294
User NOte:
Optimal dilutions for each application to be determined by the researcher.