ProSci

AGPAT1 Antibody

Product Code:
 
PSI-22-279
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot analysis of extracts of various cell lines, using AGPAT1 antibody (22-279) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 2
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug AGPAT1 antibody (22-279). Western blot was performed from the immunoprecipitate using AGPAT1 antibody (22-279) at a dilition of 1:1000.
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Western blot analysis of extracts of various cell lines, using AGPAT1 antibody (22-279) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug AGPAT1 antibody (22-279). Western blot was performed from the immunoprecipitate using AGPAT1 antibody (22-279) at a dilition of 1:1000.

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PSI-22-279-50uL50uL£433.00
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Further Information

Additional Names:
1-AGPAT1, G15, LPAAT-alpha, LPAATA, 1-acyl-sn-glycerol-3-phosphate acyltransferase alpha, 1-AGP acyltransferase 1, 1-AGPAT 1, 1-acylglycerol-3-phosphate O-acyltransferase 1 (acetoacetly Coenzyme A thiolase), 1-acylglycerol-3-phosphate O-acyltransferase 1 (lysophosphatidic acid acyltransferase, alpha), epididymis secretory sperm binding protein, lysophosphatidic acid acyltransferase alpha, lysophospholipid acyltransferase
Application Note:
WB: 1:500 - 1:2000

IP: 1:50 - 1:100
Background:
This gene encodes an enzyme that converts lysophosphatidic acid (LPA) into phosphatidic acid (PA). LPA and PA are two phospholipids involved in signal transduction and in lipid biosynthesis in cells. This enzyme localizes to the endoplasmic reticulum. This gene is located in the class III region of the human major histocompatibility complex. Alternative splicing results in two transcript variants encoding the same protein.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 204-283 of human AGPAT1 (NP_006402.1).
NCBI Gene ID #:
10554
NCBI Official Name:
1-acylglycerol-3-phosphate O-acyltransferase 1
NCBI Official Symbol:
AGPAT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 32kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
Q99943
User NOte:
Optimal dilutions for each application to be determined by the researcher.