ProSci

P4HTM Antibody

Product Code:
 
PSI-22-028
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using P4HTM antibody (22-028) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 120s.
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Western blot analysis of extracts of various cell lines, using P4HTM antibody (22-028) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 120s.

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PSI-22-028-50uL50uL£433.00
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Further Information

Additional Names:
EGLN4, HIFPH4, P4H-TM, PH-4, PH4, PHD4, transmembrane prolyl 4-hydroxylase, HIF-PH4, HIF-prolyl hydroxylase 4, HPH-4, P4H with transmembrane domain, Prolyl hydroxlase domain-containing 4, hypoxia-inducible factor prolyl 4-hydroxylase, hypoxia-inducible factor prolyl hydroxylase 4, proline 4-hydroxylase, prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum)
Application Note:
WB: 1:500 - 1:2000
Background:
The product of this gene belongs to the family of prolyl 4-hydroxylases. This protein is a prolyl hydroxylase that may be involved in the degradation of hypoxia-inducible transcription factors under normoxia. It plays a role in adaptation to hypoxia and may be related to cellular oxygen sensing. Alternatively spliced variants encoding different isoforms have been identified.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 294-563 of human P4HTM (NP_808807.2).
NCBI Gene ID #:
54681
NCBI Official Name:
prolyl 4-hydroxylase, transmembrane
NCBI Official Symbol:
P4HTM
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 55kDa
Purification:
Affinity purification
Research Area:
Cancer
Swissprot #:
Q9NXG6
User NOte:
Optimal dilutions for each application to be determined by the researcher.