ProSci

EIF2C2 Antibody

Product Code:
 
PSI-19-980
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 4
Western blot analysis of extracts of various cell lines, using AGO2 antibody (19-980) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 4
Immunohistochemistry of paraffin-embedded human stomach using AGO2 antibody (19-980) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded mouse liver using AGO2 antibody (19-980) at dilution of 1:100 (40x lens).
4 / 4
Immunohistochemistry of paraffin-embedded mouse brain using AGO2 antibody (19-980) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using AGO2 antibody (19-980) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human stomach using AGO2 antibody (19-980) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using AGO2 antibody (19-980) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using AGO2 antibody (19-980) at dilution of 1:100 (40x lens).

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PSI-19-980-50uL50uL£433.00
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Further Information

Additional Names:
CASC7, EIF2C2, LINC0098PPD, Q1protein argonaute-2, PAZ Piwi domain protein, argonaute 2, RISC catalytic component, cancer susceptibility candidate 7, cancer susceptibility candidate 7 (non-protein coding), eukaryotic translation initiation factor 2C, 2, long intergenic non-protein coding RNA 98protein slicer
Application Note:
WB: 1:500 - 1:1000

IHC: 1:50 - 1:200
Background:
This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human EIF2C2 (NP_036286.2).
NCBI Gene ID #:
27161
NCBI Official Name:
argonaute RISC catalytic component 2
NCBI Official Symbol:
AGO2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 97kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q9UKV8
User NOte:
Optimal dilutions for each application to be determined by the researcher.