ProSci

DICER1 Antibody

Product Code:
 
PSI-19-979
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using DICER1 antibody (19-979) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 5
Immunohistochemistry of paraffin-embedded human lung cancer using DICER1 antibody (19-979) at dilution of 1:100 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded human gastric cancer using DICER1 antibody (19-979) at dilution of 1:100 (40x lens).
4 / 5
Immunohistochemistry of paraffin-embedded mouse brain using DICER1 antibody (19-979) at dilution of 1:100 (40x lens).
5 / 5
Immunofluorescence analysis of L929 cells using DICER1 Polyclonal Antibody (19-979) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using DICER1 antibody (19-979) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human lung cancer using DICER1 antibody (19-979) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using DICER1 antibody (19-979) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using DICER1 antibody (19-979) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of L929 cells using DICER1 Polyclonal Antibody (19-979) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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PSI-19-979-50uL50uL£433.00
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Further Information

Additional Names:
DCR1, Dicer, Dicer1e, GLOW, HERNA, K12H4.8-LIKE, MNG1, RMSE2, endoribonuclease Dicer, Dicer1, Dcr-1 homolog, dicer 1, double-stranded RNA-specific endoribonuclease, dicer 1, ribonuclease type III, helicase MOI, helicase with RNAse motif
Application Note:
WB: 1:500 - 1:1000

IHC: 1:50 - 1:200

IF: 1:100 - 1:200
Background:
This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1200-1460 of human DICER1 (NP_085124.2).
NCBI Gene ID #:
23405
NCBI Official Name:
dicer 1, ribonuclease III
NCBI Official Symbol:
DICER1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 250kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q9UPY3
User NOte:
Optimal dilutions for each application to be determined by the researcher.