ProSci

Pan DiMethyl-lysine pAb

Product Code:
 
PSI-19-907
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 5
Western blot analysis of extracts of various cell lines, using pan-Kme2 antibody (19-907) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 5
Dot-blot analysis of all sorts of methylation peptides using Pan DiMethyl-lysine antibody (19-907) at 1:1000 dilution.
3 / 5
Immunofluorescence analysis of C6 cells using Pan DiMethyl-lysine antibody (19-907) at dilution of 1:100. Blue: DAPI for nuclear staining.
4 / 5
Immunofluorescence analysis of HeLa cells using Pan DiMethyl-lysine antibody (19-907) at dilution of 1:100. Blue: DAPI for nuclear staining.
5 / 5
Immunofluorescence analysis of NIH/3T3 cells using Pan DiMethyl-lysine antibody (19-907) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using pan-Kme2 antibody (19-907) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Dot-blot analysis of all sorts of methylation peptides using Pan DiMethyl-lysine antibody (19-907) at 1:1000 dilution.
Immunofluorescence analysis of C6 cells using Pan DiMethyl-lysine antibody (19-907) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using Pan DiMethyl-lysine antibody (19-907) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Pan DiMethyl-lysine antibody (19-907) at dilution of 1:100. Blue: DAPI for nuclear staining.

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PSI-19-907-50uL50uL£541.00
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Further Information

Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100

IP: 1:50 - 1:100
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence containing dimethylated K.
Modifications:
Methylated
NCBI Official Symbol:
pan Kme2
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 17kDa
Purification:
Affinity purification
Research Area:
Other
User NOte:
Optimal dilutions for each application to be determined by the researcher.