ProSci

PHC1 Antibody

Product Code:
 
PSI-19-894
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Immunohistochemistry of paraffin-embedded rat testis using PHC1 antibody (19-894) at dilution of 1:100 (40x lens).
2 / 3
Immunohistochemistry of paraffin-embedded human adenomyosis using PHC1 antibody (19-894) at dilution of 1:200 (40x lens).
3 / 3
Western blot analysis of extracts of various cell lines, using PHC1 Antibody (19-894) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat testis using PHC1 antibody (19-894) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human adenomyosis using PHC1 antibody (19-894) at dilution of 1:200 (40x lens).
Western blot analysis of extracts of various cell lines, using PHC1 Antibody (19-894) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.

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PSI-19-894-50uL50uL£433.00
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Further Information

Additional Names:
EDR1, HPH1, MCPH11, RAE28, polyhomeotic-like protein 1, early development regulator 1 (homolog of polyhomeotic 1), early development regulatory protein 1, polyhomeotic-like 1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene is a homolog of the Drosophila polyhomeotic gene, which is a member of the Polycomb group of genes. The gene product is a component of a multimeric protein complex that contains EDR2 and the vertebrate Polycomb protein BMH1. The gene product, the EDR2 protein, and the Drosophila polyhomeotic protein share 2 highly conserved domains, named homology domains I and II. These domains are involved in protein-protein interactions and may mediate heterodimerization of the protein encoded by this gene and the EDR2 protein.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 750-940 of human PHC1 (NP_004417.2).
NCBI Gene ID #:
1911
NCBI Official Name:
polyhomeotic homolog 1
NCBI Official Symbol:
PHC1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 125kDa/135kDa
Purification:
Affinity purification
Research Area:
Stem Cell
Swissprot #:
P78364
User NOte:
Optimal dilutions for each application to be determined by the researcher.