ProSci

SNRPE Antibody

Product Code:
 
PSI-19-697
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 5
Western blot analysis of extracts of various cell lines, using SNRPE antibody (19-697) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
2 / 5
Immunohistochemistry of paraffin-embedded human colon using SNRPE Antibody (19-697) at dilution of 1:200 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded mouse brain using SNRPE Antibody (19-697) at dilution of 1:200 (40x lens).
4 / 5
Immunohistochemistry of paraffin-embedded mouse stomach using SNRPE Antibody (19-697) at dilution of 1:200 (40x lens).
5 / 5
Immunofluorescence analysis of U2OS cells using SNRPE antibody (19-697). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using SNRPE antibody (19-697) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human colon using SNRPE Antibody (19-697) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using SNRPE Antibody (19-697) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using SNRPE Antibody (19-697) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using SNRPE antibody (19-697). Blue: DAPI for nuclear staining.

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PSI-19-697-50uL50uL£433.00
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Further Information

Additional Names:
HYPT11, SME, Sm-E, snRNP-E, small nuclear ribonucleoprotein E, sm protein E
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is a core component of U small nuclear ribonucleoproteins, which are key components of the pre-mRNA processing spliceosome. The encoded protein plays a role in the 3' end processing of histone transcripts. This protein is one of the targets in the autoimmune disease systemic lupus erythematosus, and mutations in this gene have been associated with hypotrichosis. Several pseudogenes of this gene have been identified.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-92 of human SNRPE (NP_003085.1).
NCBI Gene ID #:
6635
NCBI Official Name:
small nuclear ribonucleoprotein polypeptide E
NCBI Official Symbol:
SNRPE
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 11kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P62304
User NOte:
Optimal dilutions for each application to be determined by the researcher.