ProSci

RFC4 Antibody

Product Code:
 
PSI-19-694
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using RFC4 antibody (19-694) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
2 / 7
Immunohistochemistry of paraffin-embedded rat testis using RFC4 Antibody (19-694) at dilution of 1:100 (40x lens).
3 / 7
Immunohistochemistry of paraffin-embedded rat testis using RFC4 Antibody (19-694) at dilution of 1:100 (40x lens).
4 / 7
Immunohistochemistry of paraffin-embedded human breast cancer using RFC4 Antibody (19-694) at dilution of 1:100 (40x lens).
5 / 7
Immunofluorescence analysis of U2OS cells using RFC4 antibody (19-694). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U2OS cells using RFC4 antibody (19-694). Blue: DAPI for nuclear staining.
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Immunoprecipitation analysis of 200ug extracts of K562 cells using 1ug RFC4 antibody (19-694). Western blot was performed from the immunoprecipitate using RFC4 antibody (19-694) at a dilition of 1:1000.
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Western blot analysis of extracts of various cell lines, using RFC4 antibody (19-694) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat testis using RFC4 Antibody (19-694) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using RFC4 Antibody (19-694) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using RFC4 Antibody (19-694) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using RFC4 antibody (19-694). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using RFC4 antibody (19-694). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of K562 cells using 1ug RFC4 antibody (19-694). Western blot was performed from the immunoprecipitate using RFC4 antibody (19-694) at a dilition of 1:1000.

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CodeSizePrice
PSI-19-694-50uL50uL£433.00
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Further Information

Additional Names:
RFC4, A1, MGC27291, RFC37
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100

IP: 1:50 - 1:200
Background:
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 204-363 of human RFC4 (NP_002907.1).
NCBI Gene ID #:
5984
NCBI Official Name:
replication factor C (activator 1) 4, 37kDa
NCBI Official Symbol:
RFC4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 40kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P35249
User NOte:
Optimal dilutions for each application to be determined by the researcher.