ProSci

MAGEA1 Antibody

Product Code:
 
PSI-19-684
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of 22Rv1 cells, using MAGEA1 antibody (19-684) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 5
Immunohistochemistry of paraffin-embedded human breast cancer using MAGEA1 antibody (19-684) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver cancer using MAGEA1 antibody (19-684) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach cancer using MAGEA1 antibody (19-684) at dilution of 1:200 (40x lens).
5 / 5
Immunofluorescence analysis of HeLa cells using MAGEA1 antibody (19-684). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of 22Rv1 cells, using MAGEA1 antibody (19-684) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human breast cancer using MAGEA1 antibody (19-684) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using MAGEA1 antibody (19-684) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach cancer using MAGEA1 antibody (19-684) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using MAGEA1 antibody (19-684). Blue: DAPI for nuclear staining.

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PSI-19-684-50uL50uL£433.00
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Further Information

Additional Names:
MAGEA1, MAGE1, MGC9326, CT1.1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human MAGEA1 (NP_004979.3).
NCBI Gene ID #:
4100
NCBI Official Name:
melanoma antigen family A, 1 (directs expression of antigen MZ2-E)
NCBI Official Symbol:
MAGEA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 45kDa
Purification:
Affinity purification
Research Area:
Cancer, Immunology
Swissprot #:
P43355
User NOte:
Optimal dilutions for each application to be determined by the researcher.