ProSci

MPG Antibody

Product Code:
 
PSI-19-647
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 4
Western blot analysis of extracts of various cell lines, using MPG antibody (19-647) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 40s.
2 / 4
Immunofluorescence analysis of U2OS cells using MPG antibody (19-647). Blue: DAPI for nuclear staining.
3 / 4
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using MPG antibody (19-647). Green: GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining. RNF168 (GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
4 / 4
Immunofluorescence analysis of A549 cells using MPG antibody (19-647).
❮❯

Western blot analysis of extracts of various cell lines, using MPG antibody (19-647) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 40s.
Immunofluorescence analysis of U2OS cells using MPG antibody (19-647). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using MPG antibody (19-647). Green: GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining. RNF168 (GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
Immunofluorescence analysis of A549 cells using MPG antibody (19-647).

No additional charges, what you see is what you pay! *

CodeSizePrice
PSI-19-647-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Additional Names:
MPG, AAG, APNG, CRA36.1, MDG, Mid1, PIG11, PIG16, anpg, ADPG
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:100
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-293 of human MPG (NP_001015052.1).
NCBI Gene ID #:
4350
NCBI Official Name:
N-methylpurine-DNA glycosylase
NCBI Official Symbol:
MPG
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 37kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P29372
User NOte:
Optimal dilutions for each application to be determined by the researcher.