ProSci

AFF4 Antibody

Product Code:
 
PSI-19-432
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 1
Western blot analysis of extracts of various cell lines, using AFF4 antibody (19-432) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit._Exposure time: 30s.
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Western blot analysis of extracts of various cell lines, using AFF4 antibody (19-432) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit._Exposure time: 30s.

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PSI-19-432-50uL50uL£433.00
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This product comes from: United States.
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Further Information

Additional Names:
AF5Q31, CHOPS, MCEF, AF4/FMR2 family member 4, ALL1-fused gene from chromosome 5q31 protein, major CDK9 elongation factor-associated protein
Application Note:
WB: 1:500 - 1:2000
Background:
The protein encoded by this gene belongs to the AF4 family of transcription factors involved in leukemia. It is a component of the positive transcription elongation factor b (P-TEFb) complex. A chromosomal translocation involving this gene and MLL gene on chromosome 11 is found in infant acute lymphoblastic leukemia with ins(5;11)(q31;q31q23).
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human AFF4 (NP_055238.1).
NCBI Gene ID #:
27125
NCBI Official Name:
AF4/FMR2 family member 4
NCBI Official Symbol:
AFF4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 150kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Transcription
Swissprot #:
Q9UHB7
User NOte:
Optimal dilutions for each application to be determined by the researcher.