ProSci

COTL1 Antibody

Product Code:
 
PSI-19-411
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using COTL1 antibody (19-411) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 4
Immunohistochemistry of paraffin-embedded mouse brain using COTL1 antibody (19-411) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded rat kidney using COTL1 antibody (19-411) at dilution of 1:100 (40x lens).
4 / 4
Immunohistochemistry of paraffin-embedded human liver damage using COTL1 antibody (19-411) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using COTL1 antibody (19-411) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse brain using COTL1 antibody (19-411) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using COTL1 antibody (19-411) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver damage using COTL1 antibody (19-411) at dilution of 1:100 (40x lens).

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PSI-19-411-50uL50uL£433.00
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Further Information

Additional Names:
Coactosin-like protein, COTL1, CLP
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100
Background:
This gene encodes one of the numerous actin-binding proteins which regulate the actin cytoskeleton. This protein binds F-actin, and also interacts with 5-lipoxygenase, which is the first committed enzyme in leukotriene biosynthesis. Although this gene has been reported to map to chromosome 17 in the Smith-Magenis syndrome region, the best alignments for this gene are to chromosome 16. The Smith-Magenis syndrome region is the site of two related pseudogenes.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human COTL1 (NP_066972.1).
NCBI Gene ID #:
23406
NCBI Official Name:
Coactosin-like protein
NCBI Official Symbol:
COTL1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 14kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
Q14019
User NOte:
Optimal dilutions for each application to be determined by the researcher.