ProSci

SAP18 Antibody

Product Code:
 
PSI-19-359
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of SW480 cells, using SAP18 antibody (19-359) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
2 / 3
Immunohistochemistry of paraffin-embedded rat brain using SAP18 antibody (19-359) at dilution of 1:100 (40x lens).
3 / 3
Immunohistochemistry of paraffin-embedded mouse brain using SAP18 antibody (19-359) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of SW480 cells, using SAP18 antibody (19-359) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using SAP18 antibody (19-359) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using SAP18 antibody (19-359) at dilution of 1:100 (40x lens).

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PSI-19-359-50uL50uL£433.00
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Further Information

Additional Names:
SAP18, SAP18P, 2HOR0202
Application Note:
WB: 1:200 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation are catalyzed by multisubunit complexes. The protein encoded by this gene is a component of the histone deacetylase complex, which includes SIN3, SAP30, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. This protein directly interacts with SIN3 and enhances SIN3-mediated transcriptional repression when tethered to the promoter. A pseudogene has been identified on chromosome 2.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 21-172 of human SAP18 (NP_005861.2).
NCBI Gene ID #:
10284
NCBI Official Name:
Sin3A-associated protein, 18kDa
NCBI Official Symbol:
SAP18
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 18kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
O00422
User NOte:
Optimal dilutions for each application to be determined by the researcher.