ProSci

DNMT3A Antibody

Product Code:
 
PSI-18-975
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using DNMT3A antibody (18-975).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 5
Immunohistochemistry of paraffin-embedded rat brain using DNMT3A Antibody (18-975) at dilution of 1:100 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded human kidney using DNMT3A Antibody (18-975) at dilution of 1:100 (40x lens).
4 / 5
Immunohistochemistry of paraffin-embedded mouse heart using DNMT3A Antibody (18-975) at dilution of 1:100 (40x lens).
5 / 5
Immunofluorescence analysis of A549 cells using DNMT3A antibody (18-975).
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Western blot analysis of extracts of various cell lines, using DNMT3A antibody (18-975).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using DNMT3A Antibody (18-975) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using DNMT3A Antibody (18-975) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using DNMT3A Antibody (18-975) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using DNMT3A antibody (18-975).

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PSI-18-975-50uL50uL£433.00
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Further Information

Additional Names:
DNA (cytosine-5)-methyltransferase 3A, Dnmt3a, DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, MHsaIIIA, DNMT3A
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant protein of human DNMT3A
NCBI Gene ID #:
1788
NCBI Official Name:
DNA (cytosine-5)-methyltransferase 3A
NCBI Official Symbol:
DNMT3A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 95kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q9Y6K1
User NOte:
Optimal dilutions for each application to be determined by the researcher.