ProSci

EIF4G2 Antibody

Product Code:
 
PSI-18-858
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using EIF4G2 antibody (18-858) at 1:400 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 3
Immunohistochemistry of paraffin-embedded human stomach using EIF4G2 antibody (18-858) (40x lens).
3 / 3
Immunohistochemistry of paraffin-embedded mouse spleen using EIF4G2 antibody (18-858) (40x lens).
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Western blot analysis of extracts of various cell lines, using EIF4G2 antibody (18-858) at 1:400 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human stomach using EIF4G2 antibody (18-858) (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using EIF4G2 antibody (18-858) (40x lens).

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PSI-18-858-50uL50uL£433.00
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Further Information

Additional Names:
EIF4G2, AAG1, DAP5, FLJ41344, NAT1, p97, P97
Application Note:
WB: 1:500 - 1:1000

IHC: 1:100 - 1:200
Background:
Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 750-850 of human EIF4G2 (NP_001409.3).
NCBI Gene ID #:
1982
NCBI Official Name:
eukaryotic translation initiation factor 4 gamma, 2
NCBI Official Symbol:
EIF4G2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 102kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle
Swissprot #:
P78344
User NOte:
Optimal dilutions for each application to be determined by the researcher.