ProSci

ABCB8 Antibody

Product Code:
 
PSI-18-750
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using ABCB8 antibody (18-750) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
2 / 5
Immunohistochemistry of paraffin-embedded human liver damage using ABCB8 antibody (18-750) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using ABCB8 antibody (18-750) at dilution of 1:100 (40x lens).
4 / 5
Immunohistochemistry of paraffin-embedded mouse heart using ABCB8 antibody (18-750) at dilution of 1:100 (40x lens).
5 / 5
Immunofluorescence analysis of HeLa cells using ABCB8 Polyclonal Antibody (18-750) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using ABCB8 antibody (18-750) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human liver damage using ABCB8 antibody (18-750) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using ABCB8 antibody (18-750) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using ABCB8 antibody (18-750) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using ABCB8 Polyclonal Antibody (18-750) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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PSI-18-750-50uL50uL£433.00
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Further Information

Additional Names:
ABCB8, EST328128, M-ABC1, MABC1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This nuclear gene encodes a multi-pass membrane protein that is targeted to the mitochondrial inner membrane. The encoded protein is an ATP-dependent transporter that may mediate the passage of organic and inorganic molecules out of the mitochondria. Loss of function of the related gene in mouse results in a disruption of iron homeostasis between the mitochondria and cytosol. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 469-718 of human ABCB8 (NP_009119.2).
NCBI Gene ID #:
11194
NCBI Official Name:
ATP-binding cassette, sub-family B (MDR/TAP), member 8
NCBI Official Symbol:
ABCB8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 80kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q9NUT2
User NOte:
Optimal dilutions for each application to be determined by the researcher.