ProSci

CAMK2B Antibody

Product Code:
 
PSI-18-677
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using CAMK2B antibody (18-677) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Western blot analysis of extracts of various cell lines, using CAMK2B antibody (18-677) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.

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PSI-18-677-50uL50uL£433.00
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Further Information

Additional Names:
CAMK2B, Beta-subunit of CaMK II, Cam kinase II beta, CaMK IIbeta, CaM-kinase II beta chain, CAMKB, Beta CaMK II, CaM kinase II beta subunit, CaM kinase II subunit beta, CaM kinase IIbeta, CAM2, Camk ii beta, CAMK2, CaMKII beta, CaMK-II subunit beta, CaMKIIbeta
Application Note:
WB: 1:500 - 1:2000
Background:
The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CAMK2B (NP_742078.1).
NCBI Gene ID #:
816
NCBI Official Name:
calcium/calmodulin-dependent protein kinase II beta
NCBI Official Symbol:
CAMK2B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 51kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Immunology, Neuroscience, Signal Transduction
Swissprot #:
Q13554
User NOte:
Optimal dilutions for each application to be determined by the researcher.