ProSci

CD18 Antibody

Product Code:
 
PSI-18-552
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 5
Western blot analysis of extracts of various cell lines, using CD18 antibody (18-552) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 5
Immunohistochemistry of paraffin-embedded Rat lung using CD18 antibody (18-552) at dilution of 1:100 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded Rat spleen using CD18 antibody (18-552) at dilution of 1:100 (40x lens).
4 / 5
Immunohistochemistry of paraffin-embedded Mouse spleen using CD18 antibody (18-552) at dilution of 1:100 (40x lens).
5 / 5
Immunofluorescence analysis of RAW264.7 cells using CD18 antibody (18-552) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using CD18 antibody (18-552) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded Rat lung using CD18 antibody (18-552) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Rat spleen using CD18 antibody (18-552) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse spleen using CD18 antibody (18-552) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of RAW264.7 cells using CD18 antibody (18-552) at dilution of 1:100. Blue: DAPI for nuclear staining.

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PSI-18-552-50uL50uL£433.00
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Further Information

Additional Names:
Integrin beta-2, Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta, Complement receptor C3 subunit beta, CD18, ITGB2, CD18, MFI7
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes an integrin beta chain, which combines with multiple different alpha chains to form different integrin heterodimers. Integrins are integral cell-surface proteins that participate in cell adhesion as well as cell-surface mediated signalling. The encoded protein plays an important role in immune response and defects in this gene cause leukocyte adhesion deficiency. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 70-170 of human CD18 (NP_000202.3).
NCBI Gene ID #:
3689
NCBI Official Name:
integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
NCBI Official Symbol:
ITGB2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 100kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Immunology, Signal Transduction, Stem Cell
Swissprot #:
P05107
User NOte:
Optimal dilutions for each application to be determined by the researcher.