ProSci

PSMD2 Antibody

Product Code:
 
PSI-18-436
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using PSMD2 antibody (18-436) at 1:600 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 4
Immunohistochemistry of paraffin-embedded human liver damage using PSMD2 antibody (18-436) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded mouse heart using PSMD2 antibody (18-436) at dilution of 1:100 (40x lens).
4 / 4
Immunofluorescence analysis of HeLa cells using PSMD2 antibody (18-436). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using PSMD2 antibody (18-436) at 1:600 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver damage using PSMD2 antibody (18-436) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using PSMD2 antibody (18-436) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using PSMD2 antibody (18-436). Blue: DAPI for nuclear staining.

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PSI-18-436-50uL50uL£433.00
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Further Information

Additional Names:
PSMD2, S2, P97, TRAP2, MGC14274, proteasome (prosome, macropain) 26S subunit, non-ATPase, 2, 55.11 protein, TNFR-associated protein 2, 26S proteasome subunit p97, 26S proteasome regulatory subunit S2, 26S proteasome non-ATPase regulatory subunit 2, tumor necrosis factor receptor-associated protein 2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. In addition to participation in proteasome function, this subunit may also participate in the TNF signalling pathway since it interacts with the tumor necrosis factor type 1 receptor. A pseudogene has been identified on chromosome 1. Alternative splicing results in multiple transcript variants of this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PSMD2 (NP_002799.3).
NCBI Gene ID #:
5708
NCBI Official Name:
proteasome (prosome, macropain) 26S subunit, non-ATPase, 2
NCBI Official Symbol:
PSMD2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 108kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
Q13200
User NOte:
Optimal dilutions for each application to be determined by the researcher.