ProSci

MVP Antibody

Product Code:
 
PSI-18-427
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 5
Western blot analysis of extracts of various cell lines, using MVP antibody (18-427) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 5
Immunohistochemistry of paraffin-embedded human esophagus using MVP Antibody (18-427) at dilution of 1:100 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded human kidney cancer using MVP Antibody (18-427) at dilution of 1:200 (40x lens).
4 / 5
Immunohistochemistry of paraffin-embedded human gastric cancer using MVP Antibody (18-427) at dilution of 1:200 (40x lens).
5 / 5
Immunofluorescence analysis of U2OS cells using MVP antibody (18-427) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using MVP antibody (18-427) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human esophagus using MVP Antibody (18-427) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using MVP Antibody (18-427) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using MVP Antibody (18-427) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using MVP antibody (18-427) at dilution of 1:100. Blue: DAPI for nuclear staining.

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PSI-18-427-50uL50uL£433.00
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Further Information

Additional Names:
MVP, Major vault protein, VAULT1, LRP
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
This gene encodes the major component of the vault complex. Vaults are multi-subunit ribonucleoprotein structures that may be involved in nucleo-cytoplasmic transport. The encoded protein may play a role in multiple cellular processes by regulating the MAP kinase, JAK/STAT and phosphoinositide 3-kinase/Akt signaling pathways. The encoded protein also plays a role in multidrug resistance, and expression of this gene may be a prognostic marker for several types of cancer. Alternatively spliced transcript variants have been observed for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human MVP (NP_005106.2).
NCBI Gene ID #:
9961
NCBI Official Name:
major vault protein
NCBI Official Symbol:
MVP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 100kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
Q14764
User NOte:
Optimal dilutions for each application to be determined by the researcher.