ProSci

PLCB1 Antibody

Product Code:
 
PSI-18-420
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 5
Western blot analysis of extracts of NCI-H460 cells, using PLCB1 antibody (18-420) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 5
Immunohistochemistry of paraffin-embedded rat heart using PLCB1 antibody (18-420) at dilution of 1:100 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded human kidney cancer using PLCB1 antibody (18-420) at dilution of 1:100 (40x lens).
4 / 5
Immunohistochemistry of paraffin-embedded rat brain using PLCB1 antibody (18-420) at dilution of 1:100 (40x lens).
5 / 5
Immunofluorescence analysis of U2OS cells using PLCB1 antibody (18-420). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of NCI-H460 cells, using PLCB1 antibody (18-420) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat heart using PLCB1 antibody (18-420) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using PLCB1 antibody (18-420) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using PLCB1 antibody (18-420) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using PLCB1 antibody (18-420). Blue: DAPI for nuclear staining.

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PSI-18-420-50uL50uL£433.00
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Further Information

Additional Names:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1, PLC-154, Phosphoinositide phospholipase C-beta-1, Phospholipase C-I, PLC-I, Phospholipase C-beta-1, PLC-beta-1, PLCB1, KIAA0581
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals. This gene is activated by two G-protein alpha subunits, alpha-q and alpha-11. Two transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 917-1216 of human PLCB1 (NP_056007.1).
NCBI Gene ID #:
23236
NCBI Official Name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1
NCBI Official Symbol:
PLCB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 139kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Neuroscience, Signal Transduction
Swissprot #:
Q9NQ66
User NOte:
Optimal dilutions for each application to be determined by the researcher.