ProSci

SMAD5 Antibody

Product Code:
 
PSI-18-406
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using SMAD5 antibody (18-406) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 3
Immunohistochemistry of paraffin-embedded human lung using Smad5 antibody (18-406) at dilution of 1:100 (40x lens).
3 / 3
Immunofluorescence analysis of HeLa cells using SMAD5 Polyclonal Antibody (18-406) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using SMAD5 antibody (18-406) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human lung using Smad5 antibody (18-406) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using SMAD5 Polyclonal Antibody (18-406) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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PSI-18-406-50uL50uL£433.00
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Further Information

Additional Names:
SMAD5, DWFC, JV5-1, MADH5
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 186-465 of human SMAD5 (NP_001001420.1).
NCBI Gene ID #:
4090
NCBI Official Name:
SMAD family member 5
NCBI Official Symbol:
Smad5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 55kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction, Stem Cell
Swissprot #:
Q99717
User NOte:
Optimal dilutions for each application to be determined by the researcher.