ProSci

APBB1 Antibody

Product Code:
 
PSI-18-403
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using APBB1 antibody (18-403) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 3
Immunohistochemistry of paraffin-embedded human colon carcinoma using APBB1 antibody (18-403) at dilution of 1:100 (40x lens).
3 / 3
Immunofluorescence analysis of U2OS cells using APBB1 antibody (18-403). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using APBB1 antibody (18-403) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human colon carcinoma using APBB1 antibody (18-403) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using APBB1 antibody (18-403). Blue: DAPI for nuclear staining.

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PSI-18-403-50uL50uL£433.00
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Further Information

Additional Names:
APBB1, amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65), FE65, MGC:9072, RIR, adaptor protein FE65a2, amyloid beta A4 precursor protein-binding, member 1, stat-like protein
Application Note:
WB: 1:50 - 1:200

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is a member of the Fe65 protein family. It is an adaptor protein localized in the nucleus. It interacts with the Alzheimer's disease amyloid precursor protein (APP), transcription factor CP2/LSF/LBP1 and the low-density lipoprotein receptor-related protein. APP functions as a cytosolic anchoring site that can prevent the gene product's nuclear translocation. This encoded protein could play an important role in the pathogenesis of Alzheimer's disease. It is thought to regulate transcription. Also it is observed to block cell cycle progression by downregulating thymidylate synthase expression. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 359-708 of human APBB1 (NP_663722.1).
NCBI Gene ID #:
322
NCBI Official Name:
amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65)
NCBI Official Symbol:
APBB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 63kDa
Purification:
Affinity purification
Research Area:
Neuroscience, Signal Transduction
Swissprot #:
O00213
User NOte:
Optimal dilutions for each application to be determined by the researcher.