ProSci

CD151 Antibody

Product Code:
 
PSI-18-393
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of mouse skeletal muscle, using CD151 Antibody (18-393) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.
2 / 3
Immunohistochemistry of paraffin-embedded rat kidney using CD151 antibody (18-393) at dilution of 1:200 (40x lens).
3 / 3
Immunohistochemistry of paraffin-embedded human lung cancer using CD151 antibody (18-393) at dilution of 1:200 (40x lens).
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Western blot analysis of extracts of mouse skeletal muscle, using CD151 Antibody (18-393) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.
Immunohistochemistry of paraffin-embedded rat kidney using CD151 antibody (18-393) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using CD151 antibody (18-393) at dilution of 1:200 (40x lens).

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PSI-18-393-50uL50uL£433.00
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Further Information

Additional Names:
GP27, MER2, PETA-3, RAPH, SFA1, TSPAN24, CD151 antigen, CD151 antigen (Raph blood group), hemidesmosomal tetraspanin CD151, membrane glycoprotein SFA-1, platelet surface glycoprotein gp27, platelet-endothelial cell tetraspan antigen 3, platelet-endothelial tetraspan antigen 3, tetraspanin-24, tspan-24
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. This protein enhances cell motility, invasion and metastasis of cancer cells. Multiple alternatively spliced transcript variants that encode the same protein have been described for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 113-221 of human CD151 (NP_001034579.1).
NCBI Gene ID #:
977
NCBI Official Name:
CD151 molecule (Raph blood group)
NCBI Official Symbol:
CD151
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 28kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Immunology, Signal Transduction
Swissprot #:
P48509
User NOte:
Optimal dilutions for each application to be determined by the researcher.