ProSci

TNNC1 Antibody

Product Code:
 
PSI-18-391
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using TNNC1 antibody (18-391) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 3
Immunohistochemistry of paraffin-embedded human colon carcinoma using TNNC1 antibody (18-391) at dilution of 1:100 (40x lens).
3 / 3
Immunofluorescence analysis of U2OS cells using TNNC1 antibody (18-391). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using TNNC1 antibody (18-391) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human colon carcinoma using TNNC1 antibody (18-391) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using TNNC1 antibody (18-391). Blue: DAPI for nuclear staining.

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PSI-18-391-50uL50uL£433.00
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This product comes from: United States.
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Further Information

Additional Names:
TNNC1, CMD1Z, Cardiac troponin C, CMH13, Troponin C type 1 (slow), TN-C, TNNC, Troponin C, slow, Troponin C1, slow
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
Troponin is a central regulatory protein of striated muscle contraction, and together with tropomyosin, is located on the actin filament. Troponin consists of 3 subunits: TnI, which is the inhibitor of actomyosin ATPase; TnT, which contains the binding site for tropomyosin; and TnC, the protein encoded by this gene. The binding of calcium to TnC abolishes the inhibitory action of TnI, thus allowing the interaction of actin with myosin, the hydrolysis of ATP, and the generation of tension. Mutations in this gene are associated with cardiomyopathy dilated type 1Z.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human TNNC1 (NP_003271.1).
NCBI Gene ID #:
7134
NCBI Official Name:
troponin C type 1 (slow)
NCBI Official Symbol:
TNNC1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 16kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
P63316
User NOte:
Optimal dilutions for each application to be determined by the researcher.