ProSci

TUBB3 Antibody [5D5D2]

Product Code:
 
PSI-18-304
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
[5D5D2]
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using TUBB3 antibody (18-304) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Western blot analysis of extracts of various cell lines, using TUBB3 antibody (18-304) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.

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CodeSizePrice
PSI-18-304-50uL50uL£505.00
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Further Information

Additional Names:
beta-4, CDCBM, CFEOM3A, class III beta-tubulin, OTTHUMP00000176846, TUBB3, TUBB4, tubulin beta-3 chain, tubulin beta-4 chain, tubulin beta-III, tubulin, beta 3
Application Note:
WB: 1:500 - 1:2000
Background:
This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant protein of human TUBB3.
NCBI Gene ID #:
10381
NCBI Official Name:
tubulin, beta 3 class III
NCBI Official Symbol:
TUBB3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 55kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Neuroscience, Signal Transduction
Swissprot #:
Q13509
User NOte:
Optimal dilutions for each application to be determined by the researcher.