ProSci

Pan-AKT Antibody

Product Code:
 
PSI-18-300
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using AKT1/AKT2/AKT3 antibody (18-300) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1min.
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Western blot analysis of extracts of various cell lines, using AKT1/AKT2/AKT3 antibody (18-300) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1min.

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PSI-18-300-50uL50uL£433.00
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Further Information

Application Note:
WB: 1:500 - 1:1000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide of human AKT1/AKT2/AKT3
NCBI Gene ID #:
207/208/10000
NCBI Official Symbol:
AKT1/AKT2/AKT3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 60kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P31749/P31751/Q9Y243
User NOte:
Optimal dilutions for each application to be determined by the researcher.