ProSci

POLR2C Antibody

Product Code:
 
PSI-18-131
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot analysis of extracts of various cell lines, using POLR2C antibody (18-131) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 2
Immunoprecipitation analysis of 150ug extracts of SW620 cells using 3ug POLR2C antibody (18-131). Western blot was performed from the immunoprecipitate using POLR2C antibody (18-131) at a dilition of 1:500.
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Western blot analysis of extracts of various cell lines, using POLR2C antibody (18-131) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunoprecipitation analysis of 150ug extracts of SW620 cells using 3ug POLR2C antibody (18-131). Western blot was performed from the immunoprecipitate using POLR2C antibody (18-131) at a dilition of 1:500.

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PSI-18-131-50uL50uL£433.00
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Further Information

Additional Names:
RPB3, RPB31, hRPB33, hsRPB3, DNA-directed RNA polymerase II subunit RPB3, DNA-directed RNA polymerase II 33 kDa polypeptide, DNA-directed RNA polymerase II subunit C, RNA polymerase II subunit 3, RNA polymerase II subunit B3, RPB33, polymerase (RNA) II (DNA directed) polypeptide C, 33kDa, polymerase (RNA) II subunit C
Application Note:
WB: 1:500 - 1:2000

IP: 1:20 - 1:50
Background:
This gene encodes the third largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a cysteine rich region and exists as a heterodimer with another polymerase subunit, POLR2J. These two subunits form a core subassembly unit of the polymerase. A pseudogene has been identified on chromosome 21.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-275 of human POLR2C (NP_116558.1).
NCBI Gene ID #:
5432
NCBI Official Name:
RNA polymerase II subunit C
NCBI Official Symbol:
POLR2C
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 38kDa
Purification:
Affinity purification
Research Area:
Neuroscience
Swissprot #:
P19387
User NOte:
Optimal dilutions for each application to be determined by the researcher.