ProSci

PIK3R3 Antibody

Product Code:
 
PSI-16-914
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using PIK3R3 antibody (16-914) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 3
Immunofluorescence analysis of NIH-3T3 cells using PIK3R3 antibody (16-914) at dilution of 1:100. Blue: DAPI for nuclear staining.
3 / 3
Immunofluorescence analysis of NIH-3T3 cells using PIK3R3 antibody (16-914) at dilution of 1:100. Blue: DAPI for nuclear staining.
❮❯

Western blot analysis of extracts of various cell lines, using PIK3R3 antibody (16-914) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of NIH-3T3 cells using PIK3R3 antibody (16-914) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using PIK3R3 antibody (16-914) at dilution of 1:100. Blue: DAPI for nuclear staining.

No additional charges, what you see is what you pay! *

CodeSizePrice
PSI-16-914-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Additional Names:
PIK3R3, DKFZp686P05226, FLJ41892, p55-GAMMA, p55
Application Note:
WB: 1:500 - 1:1000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
Phosphatidylinositol 3-kinase (PI3K) phosphorylates phosphatidylinositol and similar compounds, which then serve as second messengers in growth signaling pathways. PI3K is composed of a catalytic and a regulatory subunit. The protein encoded by this gene represents a regulatory subunit of PI3K. The encoded protein contains two SH2 domains through which it binds activated protein tyrosine kinases to regulate their activity.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant protein of human PIK3R3
NCBI Gene ID #:
8503
NCBI Official Name:
phosphoinositide-3-kinase, regulatory subunit 3 (gamma)
NCBI Official Symbol:
PIK3R3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 60kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Immunology, Signal Transduction
Swissprot #:
Q92569
User NOte:
Optimal dilutions for each application to be determined by the researcher.