ProSci

SRSF1 Antibody

Product Code:
 
PSI-16-628
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 5
Western blot analysis of extracts of various cell lines, using SRSF1 antibody (16-628) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 5
Immunohistochemistry of paraffin-embedded rat lung using SRSF1 Antibody (16-628) at dilution of 1:100 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded human lung cancer using SRSF1 Antibody (16-628) at dilution of 1:100 (40x lens).
4 / 5
Immunohistochemistry of paraffin-embedded mouse esophagus using SRSF1 Antibody (16-628) at dilution of 1:100 (40x lens).
5 / 5
Immunofluorescence analysis of U2OS cells using SRSF1 antibody (16-628). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using SRSF1 antibody (16-628) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat lung using SRSF1 Antibody (16-628) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using SRSF1 Antibody (16-628) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse esophagus using SRSF1 Antibody (16-628) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using SRSF1 antibody (16-628). Blue: DAPI for nuclear staining.

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PSI-16-628-50uL50uL£433.00
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Further Information

Additional Names:
SFRS1, ASF, SF2, SFRS1, SF2p33, SRp30a
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a member of the arginine/serine-rich splicing factor protein family. The encoded protein can either activate or repress splicing, depending on its phosphorylation state and its interaction partners. Multiple transcript variants have been found for this gene. There is a pseudogene of this gene on chromosome 13.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-248 of human SRSF1 (NP_008855.1).
NCBI Gene ID #:
6426
NCBI Official Name:
serine/arginine-rich splicing factor 1
NCBI Official Symbol:
SRSF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 30kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q07955
User NOte:
Optimal dilutions for each application to be determined by the researcher.