ProSci

SIN3A Antibody

Product Code:
 
PSI-16-224
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using SIN3A antibody (16-224) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 3
Immunofluorescence analysis of U2OS cells using SIN3A antibody (16-224). Blue: DAPI for nuclear staining.
3 / 3
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug SIN3A antibody (16-224). Western blot was performed from the immunoprecipitate using SIN3A antibody (16-224) at a dilition of 1:500.
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Western blot analysis of extracts of various cell lines, using SIN3A antibody (16-224) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of U2OS cells using SIN3A antibody (16-224). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug SIN3A antibody (16-224). Western blot was performed from the immunoprecipitate using SIN3A antibody (16-224) at a dilition of 1:500.

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PSI-16-224-50uL50uL£433.00
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Further Information

Additional Names:
SIN3A, DKFZP434K2235, FLJ90319, KIAA0700,
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200

IP: 1:50 - 1:100

ChIP: 1:20 - 1:100
Background:
The protein encoded by this gene is a transcriptional regulatory protein. It contains paired amphipathic helix (PAH) domains, which are important for protein-protein interactions and may mediate repression by the Mad-Max complex.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human SIN3A (NP_056292.1).
NCBI Gene ID #:
25942
NCBI Official Name:
SIN3 transcription regulator family member A
NCBI Official Symbol:
SIN3A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 165kDa
Purification:
Affinity purification
Research Area:
Cancer, Neuroscience
Swissprot #:
Q96ST3
User NOte:
Optimal dilutions for each application to be determined by the researcher.