ProSci

SLC6A3 Antibody

Product Code:
 
PSI-15-942
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot analysis of extracts of various cell lines, using SLC6A3 antibody (15-942) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
2 / 2
Immunohistochemistry of paraffin-embedded rat brain using SLC6A3 antibody (15-942) at dilution of 1:200 (40x lens).
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Western blot analysis of extracts of various cell lines, using SLC6A3 antibody (15-942) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat brain using SLC6A3 antibody (15-942) at dilution of 1:200 (40x lens).

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PSI-15-942-50uL50uL£433.00
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Further Information

Additional Names:
SLC6A3, solute carrier family 6 member 3, solute carrier family 6 (neurotransmitter transporter, dopamine), member 3, sodium-dependent dopamine transporter, DAT1, DAT, DA transporter
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100
Background:
This gene encodes a dopamine transporter which is a member of the sodium- and chloride-dependent neurotransmitter transporter family. The 3' UTR of this gene contains a 40 bp tandem repeat, referred to as a variable number tandem repeat or VNTR, which can be present in 3 to 11 copies. Variation in the number of repeats is associated with idiopathic epilepsy, attention-deficit hyperactivity disorder, dependence on alcohol and cocaine, susceptibility to Parkinson disease and protection against nicotine dependence.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 500 to the C-terminus of human SLC6A3 (NP_001035.1).
NCBI Gene ID #:
6531
NCBI Official Name:
solute carrier family 6 (neurotransmitter transporter), member 3
NCBI Official Symbol:
SLC6A3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 90kDa
Purification:
Affinity purification
Research Area:
Neuroscience
Swissprot #:
Q01959
User NOte:
Optimal dilutions for each application to be determined by the researcher.